Fig. 3. Disruption of the microtubule network inhibits nuclear accumulation of PrP. (A) ScN2a cells were treated overnight with increasing concentrations of colchicine. Total and PK treated cell lysates and purified nuclei were analyzed by western blot with anti PrP antibodies. (B) Isolated nuclei from ScN2a cells (a-f) or whole cells (g-l), were either non-treated (control) or treated overnight with 10 µg/ml colchicine and analyzed by immunofluorescence as described in Fig. 2. Bar, 8 µm