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Fig. 8. In search of evidence for the existence of ALT in HUVEC subjected to chronic oxidative stress. Indirect immunofluorescence staining of TRF1 (green) and PML protein (red) in late passage HUVEC grown under pro-oxidant conditions (BSO) and in U2OS cells. Co-localisation of TRF1 and PML appears yellow (arrows). DNA was counterstained with DAPI (blue).