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Fig. 2. Cell division is delayed in flp1{Delta} mutant S. pombe cells. nda3-KM311 and nda3-KM311 flp1{Delta} (otherwise isogenic strains) were blocked in metaphase by incubation of asynchronously growing cultures (Asn) at 20°C for 6 hours. Cells were then released from the block at 32°C and samples were taken at the indicated intervals and processed for cytological and biochemical analysis. (A) Plots of binucleate (top plot, Mitotic Index), septated cells (middle plot, Septation Index) and relative kinase activity (lower plot) are shown. (B) Cdc13p-associated H1 kinase activity (top panels) and relative amounts of immunoprecipitated Cdc2 for the kinase assays (lower panels). (C) Cdc13p, Cdc25p and {alpha}-tubulin western analysis (as indicated) from samples processed from nda3-KM311 and nda3-KM311 flp1{Delta} cells. (D) Elutriated G2 nda3-KM311 (control) or nda3-KM311 flp1{Delta} cells were blocked at metaphase by incubating them for 4 hours at 20°C and then released at 32°C. Samples were taken at the indicated intervals and processed for {alpha}-tubulin and Cdc25p western analysis.