Fig. 10. Cross-linking of connexins in HeLa-Cx43/Cx26 cells untreated or treated with brefeldin A for 4 hours or 20 hours. Triton X-100 soluble extracts (containing connexons) were reacted with disuccinimyl suberate (DSS) cross-linker dissolved in DMSO (+) or were incubated with DMSO solvent alone (–). Samples containing 100 µg protein were resolved by SDS-PAGE, and Cx26 (A) or Cx43 (B) were detected by immunoblot analysis using anti-Cx26 or anti-Cx43 antibodies. Migration of molecular mass markers is indicated to the left of the blot.