Fig. 2. Detection of the distributions of Cx26 (red) and Cx43 (green) in HEKCx26 cells (A-H) or Cx40 (red) and Cx43 (green) in HeLa-Cx43/Cx40 cells (I-K) by immunofluorescence microscopy. (A-C) Conventional immunofluorescence images of cells double labeled for Cx26 (shown alone in B) and for Cx43 (shown alone in C). Merged image of both immunoreactivities is shown in A. (D) Stacked z-series from double label confocal analysis of a single large plaque. (E-H) Z-series of slices obtained at different depths (separated by 0.3-1.2 µm) from confocal analysis of plaques in HEK-Cx26 cells. (I-K) Z-series of slices similarly obtained at different depths from plaques in pairs of HeLa-Cx43/Cx40 cells where simultaneously obtained images show precise co-localization of Cx43 (red) and Cx40 (green). Bar: 27 µm (A-C); 1 µm (D); 5 µm (E-H); 3 µm (I,J).