Fig. 9. Immuno-gold localization of protein 4.1 epitopes on the PLF network. (A) Western blot of manually isolated Xenopus oocyte nuclei probed with antibodies against protein 4.1 shows a major isoform of 80 kDa, plus potential minor isoforms visible only upon longer exposure (data not shown). (B,C) FeSEM images of different PLFs showing protein 4.1 labeled with primary antibodies plus 10-nm-gold-labeled secondary antibodies. Gold particles were visualized directly in backscatter images (not shown), and are pseudo-colored yellow.