Fig. 6. Me(3)K20H4 localises to the centromeric and telomeric heterochromatin during spermatogenesis and transiently accumulates at the PAR region. Me(3)K20H4 distribution (green) is followed in pachytene and diplotene nuclei. The axial elements of the paired chromosomes are visualised with anti-SYCP3 antibodies (red) and the centromeres with human anti-centromeric antibodies (ACA; blue). During early (A-C) and mid-pachytene (D-F), Me(3)K20H4 localises at the centromeres, some of the telomeres (arrows in C and F) and the PAR region of the XY-body. The centromeric heterochromatin of one of the autosomes (asterisk) and of the Y chromosome are not stained with anti Me(3)K20H4 antibodies. At late pachytene (G-I) and diplotene (J-L) the PAR (arrow indicates the PAR) and telomeric staining of Me(3)K20H4 have disappeared.