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Fig. 4. Active Aurora-A and serine 353-phosphorylated CDC25B localise at prophase and metaphase centrosomes. (A) HeLa cells were fixed and subjected to double immunofluorescence staining with SE96 polyclonal and Aurora-A monoclonal antibodies; SE96 (green), Aurora-A (red) and DAPI (blue). Photomicrographs of cells representative of each stage of mitosis are shown. Interphase (A), prophase (B), prometaphase (C), metaphase (D), anaphase A (E) and anaphase B (F). (B) HeLa cells were fixed and stained with monoclonal Aurora-A antibodies and polyclonal antibodies against threonine 288-phosphorylated Aurora-A. Selected cell cycle phases are shown.