Fig. 6. In situ localization of cells expressing the CD36/LIMPII receptor in tissue of S. domuncula. Cryosections of tissue were hybridized with DIG-labeled SDCD36/LIMPII. Subsequently, the specimens were incubated with anti-digoxigenin/alkaline phosphatase and the signals were detected with NBT/X-phosphate as described under Materials and Methods. (A,C) hybridization with antisense SDCD36/LIMPII; (B,D) hybridization with sense SDCD36/LIMPII. Canals (c) of the aquiferous system within the mesohyl (m) are shown. The canals are lined up by an epithelial layer formed from pinacocytes, which are SDCD36/LIMPII-positive cells. Magnifications: (A,B) x50; (C,D) x100.