Fig. 2. Ca²⁺ responses of soybean cells to 50 µM CPA treatment. (A) Black trace, [Ca²⁺]_cyt elevations induced by CPA in soybean cells grown in normal culture medium; grey trace, [Ca²⁺]_cyt elevations induced by CPA in soybean cells incubated in Ca²⁺-free medium containing 100 µM EGTA. (B) Black trace, [Ca²⁺]_cyt elevations induced by CPA in soybean cells grown in normal culture medium; grey trace, [Ca²⁺]_cyt elevations induced by CPA in soybean cells pre-treated for 1 hour with 200 nM bafilomycin A₁. Traces are representative of three independent experiments, which gave very similar results. Arrow, time of CPA administration.