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Fig. 8. Changes in nuclear morphology in response to CPA treatment. Nuclei of 24-hour CPA-treated cells were stained with Hoechst 33342 and propidium iodide and processed for fluorescent microscopy. (A-C) Untreated cells. (D-I) 50 µM CPA-treated (24 hours) cells. (E,F) Early PCD nucleus. (H,I) Late PCD nuclei. Pictures represent typical examples. cc, chromatin condensation; cw, cell wall; nu, nucleus; pm, plasma membrane. The arrow indicates the detachment of the plasma membrane from the cell wall in CPA-treated cells.