Fig. 1. Maintenance of morphology and differentiation in organotypic 3D-cocultures in the presence of laminin 1-fragment (Lf). Histology of epithelia at 14 (A,B) and 19 days (C,D), grown without (A,C) and with Lf (B,D) reveals comparable stratification including defined granular (arrows) and cornified layers (SC), but an increased tendency for splitting at the tissue interface with Lf (D; arrowhead). (E-H), Analysis of Lf-treated tissue by indirect immunofluorescence (IIF) indicates a largely regular expression of the early epidermal differentiation markers keratin K1 and K10 (E) as well as the intermediate and late markers K2e (F), epidermal transglutaminase (G), and loricrin (H). Scale bars: 50 µm.