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Fig. 3. Dissociation of assembled BM components by delayed L{gamma}f treatment (starting at day 10) and analysed at day 14. Nidogen (A) and BM laminin (C; detection of {gamma}1 chain) are almost absent after this short treatment, whereas the linear deposition of both type IV collagen (B; but some diffuse dislocation into gel matrix) and laminin-5 (D; {gamma}2 chain) persists. Scale bars: 50 µm.