Fig. 7. Localisation of BM components and adhesion molecules in control (left column) and Lf-treated epithelia (right column) at 14 days. The control shows a band of denser gold labelling for type IV collagen (A) which becomes extensively dispersed and dislocated in the presence of Lf (B; larger area, about two-fold lower magnification). Similar effects are seen for laminin-5 (C, D; 10 nm gold) which by double labelling also reveals a looser association of the integrin ß4 chain than in controls (C versus D; 5 nm gold). In addition, and in contrast to regular clusters of bullous pemphigoid antigen 1 (E; BPAG1/BP230; 10 nm), only a few single gold particles are seen in Lf-treated specimens (F; outside the field, vast areas without any label). Consequently keratin-positive filaments (`pan'-keratin; 10 nm), normally attached to the hemidesmosomes (G), are, in treated samples (H), largely retracted from the basal cell pole, which is partially demarcated by ß4 (5 nm). In A and B 1 nm gold particles were used together with silver enhancement. e, epithelium; m, extracellular matrix. Scale bars: 150 (A,C-H) and 300 nm (B).