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Fig. 8. Protein analysis by western blotting of the separated epithelial and `dermal' compartments (collagen matrix with resident fibroblasts). Extracts were prepared from 3D-cocultures (–/+ L{gamma}f treatment as indicated) grown for 12 (left panel) or 14 (right panel) days at the air-liquid interface. Reactions with antibodies against the laminin {alpha}5 (L{alpha}5) and {gamma}1 chain (L{gamma}1), and against nidogen (ND) are shown, the lower part of third blot (*) demonstrates comparable sample loading. The size of the bands of presumptive BM components is indicated in kDa.