Fig. 3. Rapamycin induces the localization of Rab7T22N to autophagic vacuoles. CHO cells expressing EGFP-Rab7T22N were incubated at 37°C for 2 hours in -MEM (control cells) or EBSS medium (starved cells) in the presence of 50 ng/µl rapamycin. Cells were briefly washed with PBS, labeled with MDC and immediately analyzed by fluorescence microscopy as described in Fig. 1.