Fig. 6. The mitochondrial uncoupler CCCP decreases both kinesin- and dynein-mediated transport but does not lead to a transient rise in retrograde transport. (A) Kymograph of distribution and movements of Mitotracker-labeled mitochondria. The position and time of drug application (1 mM CCCP and Lucifer Yellow in DMEM) is shown in blue. (B) Hand-drawn traces of anterograde (blue) and retrograde (red) mediated movements used to quantify the mitochondrial movements. (C-F) Normalized anterograde and retrograde mediated transport. Error bars are the normalized 95% confidence intervals (n=5 experiments). (D,E) There is significant decrease in both anterograde and retrograde mediated transport. (F) After washout, transport recovers.