Fig. 7. YFP-GATE16 is present on autophagosome membranes. F9 cells stably transfected with YFP-GATE16 were cultured in Hanks' solution for 2 hours and fixed. The localization of YFP-GATE16 was examined by silver-enhanced immunogold electron microscopy using anti-GFP antibody. The isolation membrane (arrow), autophagosomes (open arrowheads) and autolysosomes (closed arrowheads) are indicated (A). The typical images of isolation membrane (B), autophagosome (C) and autolysosome (D) are shown at higher magnification. Scale bar, 1 µm.