Fig. 4. Immunohistochemistry for c-Kit and ryanodine receptors. Smooth muscle (including the myenteric plexus) of mouse small intestine was double-labelled with anti-c-Kit antibody (A: ACK2 with Alexa 488, red) and anti-ryanodine receptor antibody (B: C34 with Alexa 594, green). C shows a merged image. Note that network-like cells were mostly stained in yellow, indicating that ICCs contain ryanodine receptors.