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Fig. 7. Analysis of angiogenesis in xenografted tumours induced by MDCK-Snail and MCDK-E47 cells. (A) Tumours induced in nude mice by MDCK-Snail (Snail) (a-d) and MDCK-E47 (E47) (e-h) cells were analysed by immunostaining for CD-31 (a,e) and endoglin (b,f); or immunohistochemistry for {alpha}-smooth muscle actin ({alpha}SMA) (c,d,g,h). Note the higher number of small blood vessels in MDCK-E47 derived tumours (e-h) than those induced by MDCK-Snail cells (a-d). (B) Expression of angiogenic factors in MDCK-Snail and MDCK-E47 induced tumours. Vibratome sections were processed either for TGFß1 mRNA (a,c) detection or for VEGF-A mRNA plus {alpha}SMA (b,d) detection. TGFß1 mRNA expression appears in both types of tumour with a scattered pattern covering the full extent of the tumour. VEGF-A expression appears only in Snail-induced tumours, in the form of isolated patches, which are also positive for {alpha}SMA. Bars, 50 µm.