Fig. 6. Effect of PKC downregulation or PKC inhibition on ALCAM-affinity. Soluble ALCAM-Fc-binding assay with KG1 cells (A) and K562-ALCAM (B) either cultured for 16 hours with () or without () 50 nM PMA or pre-incubated for 10 minutes at 37°C with chelerythrine chloride (5 µM, ) or myristoylated PKC pseudosubstrate (100 µM, ). Cells are incubated with increasing concentrations of soluble ALCAM-Fc and the percentage of cells that have bound ligand is determined by flow cytometry. Data are representative of three independent experiments.