Fig. 3. Inhibition of glucose trimming does not destabilize the wild-type Shaker protein. After a 30 minute pulse, cells were harvested immediately (0 h) or after a 24 hour chase period. The amount of Shaker protein remaining at 24 hours was 86% (-dNJ), 81% (+dNJ) or 81% (+dNJ^*). The parenthetical designations have the same meaning as in Fig. 2. Open and closed arrowheads indicate the positions of the immature and mature forms of the Shaker protein, respectively. The open circle indicates the position of a nonspecific background band of variable intensity, which is also seen in untransfected cells upon immunoprecipitation with the Shaker antiserum (data not shown). A representative fluorograph is shown (n=3).