Fig. 5. Wild-type Shaker is destabilized when calnexin interaction and ER exit are blocked. (A) Shaker-expressing cells were treated with BFA/NOC and dNJ (1 mM) added during the starvation, pulse and chase periods; or with BFA/NOC, dNJ and Lac (10 µM). Cells were labeled for 10 minutes and chased for 0, 24 or 48 hours as indicated. Open arrowhead indicates the position of the core-glycosylated, immature form of the Shaker protein. (B) The amount of core glycosylated Shaker protein remaining at 48 hours of chase was quantified by densitometry and expressed relative to the amount present at 0 time of chase (100%). The number of experiments for each condition (n) is shown in parentheses on the appropriate bar.P<0.0001 compared with BFA/NOC; P<0.0001, compared with BFA/NOC+dNJ (Student's t-test).