Fig. 6. Inhibition of chemotaxis by LY294002. Wild-type cells were starved for 5 hours on plates in phosphate buffer, resuspended in buffer and deposited as small droplets on hydrophobic agar containing different concentrations of LY294002. After about 15 minutes, droplets with different cAMP concentrations were deposited close to the amoebae and the chemotaxis response was observed during the following 30 minutes. The data are presented as a percentage of the populations (about 20 for each cAMP concentration) that showed a positive chemotactic response.