Fig. 5. Albino Tyr(C85S) forms aggregates in the absence of glucose trimming. After a 1-hour incubation in the presence or absence of DNJ, Tyr and Tyr(C85S) melanocytes were lysed in 2% CHAPS, separated on a 5-25% linear sucrose gradient by ultracentrifugation, and subjected to immunoblotting as previously described. The stacking gels were included in the autoradiography.