Fig. 7 . BiP and PDI, but not calnexin, colocalize with aggregates of triglucosylated Tyr(C85S). (A) After a 4-hour incubation in the presence or absence of 500 µM DNJ, wild-type Tyr and (B) mutant Tyr(C85S) mouse melanocytes were fixed, permeabilized and labeled with anti-tyrosinase (green), anti-BiP, anti-PDI, or anti-calnexin antibodies (red) as indicated. Colocalization is indicated by yellow coloration. Bar, 2 µm.