Fig. 4. F cells expressing the mutant L1106A migrate slower in wound healing assays. L1106A expression inhibits F cell migration compared with cells that express vector, WT or the mutant K634R F in wound healing assays. Upon wounding, F cell monolayers were incubated in DMEM supplemented with 0.2% FBS and analyzed for their ability to repopulate the wounded area. Phase-contrast images were taken at 10 and 22 hours after wounding. Assays were conducted at least three times and yielded similar results.