Fig. 2. Immunofluorescence labeling of HTR12 and FISH detection of 180 bp repeats and Athila. (A-C) Immunosignals of HTR12 (red), FISH signals of 180 bp repeats (blue) and Athila (green) on prometaphase chromosomes (A), metaphase chromosomes (B) and in an interphase nucleus (C). Scale bars: 5 µm. (D-F) HTR12, 180 bp and Athila signals on the three (as in A-C) chromosomes, and the superimposed images. Scale bars: 1 µm. (G,H) HTR12 (red) and 180 bp repetitive sequence (blue) on two different sized prometaphase chromosomes (G) and in an interphase nucleus (H). Arrows indicate completely overlapping signals. Scale bars: 1 µm.