Fig. 3. Immunofluorescence labeling of phosphorylated histone H3 and HTR12 on putative dicentric chromosomes. Chromosomes were counterstained with DAPI (blue). (A) Immunosignals from phosphorylated histone H3 (red), (B) FISH signals from 180 bp repetitive sequence (green) on a dicentric chromosome, (C) superimposition of A and B. Scale bar: 2 µm. (D) A metaphase cell containing a putative dicentric chromosome (arrow). HTR12 and two 180 bp repeat sites appear as red and green, respectively. Scale bar: 2 µm. (E) An anaphase cell with chromatin bridges. Arrows indicate HTR12 signals (red) and FISH signals of 180 bp repeats (green). Scale bar: 5 µm.