Fig. 4. Immunofluorescence labeling of HTR12, and FISH detection of 180 bp repetitive and Athila sequences on extended chromatin fibers. (A) Immunosignals from HTR12 (red) on DAPI-staining extended chromatin fibers (white). (B) FISH signals from 180 bp repeats (green). (C) Superimposition of the HTR12 signals in A and the image in B, with overlapping regions shown in yellow. (D) FISH signals from Athila (blue). (E) Superimposition of HTR12 signals in A and the image in D. (F) Superimposition of 180 bp repeat signals in B and the image in E. Scale bar: 5 µm. (G) Immunosignals from HTR12 and FISH signals from 180 bp repeats on fully extended chromatin fibers. The knobs of 180 bp repeats are colocalized (yellow) with immunosignals of HTR12. Scale bar: 5 µm.