Fig. 2. Characterization of MIAMI cells. (A) MIAMI cells were harvested and labeled with antibodies against CD29, CD36, CD49E, CD54, CD56, CD63, CD81, CD90, CD122, CD164, CNTFR, and HLA-DR or control IgGs as indicated and analyzed by FACS. Plots show isotype control IgG-staining profiles (black lines) versus specific antibody staining profile (colored lines). (B) Ten micrograms of total protein was analyzed by western blot using an antibody that recognizes human cMet. Expression of the 190 kDa precursor and 140 kDa cleaved receptor was detected in MIAMI cells and in the PC-3 prostate cancer cell line (positive control). (C,D) RT-PCR analysis of expression of the stem cell markers Oct-4 and Rex-1 (C) in MIAMI cells and of hTERT mRNA in two separate MIAMI cell isolates (D, RNA isolated from the human foreskin fibroblast cell line hTERT-BJ1 was used as positive control; +ve cont). PCR products of specific sizes corresponding to each gene were obtained. RNA-specific amplification is demonstrated by the absence of a band when RT was excluded (- RT).