Fig. 2. Osh protein depletion caused defective chitin accumulation. Calcofluor staining of yeast cells viewed by fluorescence microscopy shows chitin deposition. (A) Chitin bud scars are visible in wild-type cells (SEY6210) at the mother-bud junction or immediately adjacent, which reflects the axial pattern of haploid budding. (B) In osh P^MET3-OSH2 cells (JRY6326) after methionine repression of OSH2 expression, Osh depletion caused chitin to accumulate at abnormal sites other than just the mother-bud junction (e.g. bud tip). Arrows indicate abnormal deposits of chitin. Exposure times for osh P^MET3-OSH2 cells were shorter that wild type indicating a comparatively greater amount of chitin.