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Fig. 5. Secretion of Gas1p to the plasma membrane was unaffected by OSH mutants. Autoradiograph of Gas1p immunoprecipitations from wild-type (W.T.; SEY6210), osh{Delta} osh4-1 (CBY886), osh{Delta} OSH4 (JRY6320) and sec18(ts) (JRY4130) cell lysates following SDS-PAGE. (p)Gas1p, precursor form of Gas1p; (m)Gas1p, mature plasma membrane form of Gas1p. After an incubation of 1 hour at 37°C, cells were pulse-labeled for 5 minutes and chased for the periods indicated (in minutes) at 37°C.