Fig. 5. Localization of GFP-SBP1 to the E235Q compartments requires C terminus coiled-coil domain. HeLa cells transfected with plasmids encoding GFP-SBP1 deletion mutants and SKD1(E235Q) were subjected to immunofluorescence analysis using anti-SKD1 antibody. Left column (black and white), GFP fluorescence micrographs of the cells transfected with GFP-SBP1 (1-309) (A), C (1-129) (B) and N (198-309) (C). Right three columns, GFP (green), Alexa594 fluorescence (red) and the merged images were obtained from the cells double transfected with SKD1(E235Q) and full-length GFP-SBP1 (1-309) (A), C (1-129) (B) and N (198-309) (C), respectively. Notice, full-length and N SBP1 were redistributed to the E235Q compartments, while C mutant was not. Bar, 20 µm.