(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. 14-3-3ß, 14-3-3{epsilon}, and 14-3-3{sigma} bind to CDC25B in transfected cells. HEK293 (left panels) or U2OS (right panels) cells were transfected with FLAG-tagged CDC25B together with either empty vector or one of the myc-tagged 14-3-3 subtypes as described in Materials and Methods. (Top row) Lysate. Expression of CDC25B and 14-3-3-subtypes was confirmed in cell lysates with anti-FLAG antibody against CDC25B or anti-myc antibody against 14-3-3, respectively. (Middle row) IP: CDC25B ({alpha}-FLAG Ab). CDC25B was immunoprecipitated with anti-FLAG beads followed by western blotting and detection with anti-FLAG antibody to detect CDC25B and anti-myc antibody to detect CDC25B-bound 14-3-3. (Bottom row) IP: 14-3-3 ({alpha}-myc Ab. Reciprocal immunoprecipitation; CDC25B was detected in anti-myc immunoprecipitates. Protein 14-3-3 subtypes were immunoprecipitated with anti-myc antibody; the collected 14-3-3 or 14-3-3-bound CDC25B was detected by immunoblotting. v, empty vector; ß, 14-3-3ß, {epsilon}; 14-3-3{epsilon}, {sigma}; 14-3-3{sigma}.