Fig. 3. Efficient binding of 14-3-3 to CDC25B requires two independent sites. HEK293 cells were co-transfected with 14-3-3 and a series of CDC25B mutants. Protein expression (Lysate) and protein binding (IP: CDC25B (-FLAG Ab)) were detected. The letters in the upper panel of Lysate indicate CDC25B wild type and respective mutants. The definitions of the abbreviations for each mutant are described in the text.