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Fig. 7. Binding of 14-3-3ß to CDC25B efficiently slowed down the nuclear import of CDC25B induced by leptomycin B (LMB). (A) HEK293 cells transfected with wild-type CDC25B and treated with 20 ng/ml LMB at for 3 hours. Transfected CDC25B was detected with anti-FLAG antibody. The upper and lower panels show the results without or with LMB treatment, respectively. (B) HEK293 cells transfected with wild-type CDC25B alone or with 14-3-3ß. Twenty-four hours after transfection, 20 ng/ml LMB were added to the cultures and the percentage of cells exhibiting nuclear-specific localization of CDC25B was determined at the indicated time-points: 0 (before addition of LMB), 1.5, 3 or 6 hours after the addition. Over 200 cells expressing CDC25B were counted to determine the percentage of cells that express CDC25B. The percentage of cells with a nuclear localization (as shown in Fig. 4) was determined from three independent experiments. , CDC25B; {circ},CDC25B with 14-3-3ß.