Fig. 2. Further localization of interaction domains on both PHB2 and Akt. Different deletion fragments of the PHB2 (A) or Akt (B) gene were separately cloned into pGADT7 and pGBKT7, respectively. The resulting clones were tested for their mutual interaction using yeast two-hybrid assays. The black bar represents the Akt-interacting region on PHB2. Abbreviations: CT, C terminus; PH, pleckstrinhomology domain. The plus and minus signs denote growth and no growth, respectively, on SD/-his/-ade/-leu/-trp plates. The numbers represent the position of amino acids in either PHB2 or Akt.