Fig. 5. Stable expression of PHB2 in C2C12 inhibits muscle differentiation. Near-confluent stable C2C12 cells expressing either an empty vector or PHB2 were allowed to differentiate in DM for the indicated times. (A) Cells were harvested and 30 µg WCEs were resolved by SDS-PAGE and subjected to immunoblotting. (B) After 24 hours in DM, cells were fixed, subjected to immunostaining and microscopic imaging. Abbreviations: DM, differentiation medium; GM, growth medium; MHC, myosin heavy chain; Phase, phase contrast.