Fig. 2. P35 expression protects activated RAW 264.7 cells from undergoing NO-mediated oligonucleosomal DNA fragmentation. RAW 264.7 cells or vector- or p35-transfected clones (1x10⁶ cells/well) in 6-well tissue culture plates were treated with cisplatin (2 µg/ml) and IFN- (25 U/ml) or LPS (1 µg/ml) and IFN- (25 U/ml) in the presence or absence of L-NMMA (1 mM) as indicated. Cells were also treated directly with NO donor, SNP (1 mM). After 36 hours of treatment, cells were assayed for DNA fragmentation using a radioisotope release assay (A) and agarose gel electrophoresis (B) as described in Materials and Methods. Data shown are mean±s.e.m. and are the representative of three independent experiments done in triplicates. ^*P<0.05 versus values of control cultures. ^**P<0.05 versus values of cisplatin and IFN-/LPS and IFN--treated cultures. ^#P<0.05 versus values for cisplatin + IFN-/LPS + IFN- or SNP treated RAW 264.7/pCMV-FLAG cells.