Fig. 2. HeLa cells microinjected with either wild-type (left column) or mutated (right column) peptide-BSA FITC conjugates (green), and then counterstained with DAPI (blue). A total of 20-50 cells were successfully microinjected per peptide and similar results were seen in all cells. The bottom of the figure presents HeLa cells that were microinjected with wild-type peptide-BSA-FITC conjugates in the presence of 2 ng/ml LMB (leptomycin B). Panels showing mutated peptide BSA-NES 1054-66 are at a higher magnification than the other micrographs.