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Fig. 3. Western blot of full-length FLAG-topo II{alpha} protein. HeLa cells were transfected with plasmid containing FLAG-topo II{alpha} plasmid via cationic lipid, and harvested after 20 hours. Protein extracts from 2x105 cells per lane were separated on a SDS-PAGE gel, blotted onto nitrocellulose and probed with FLAG M2 antibody. Lane A is protein from cells transfected with non-mutated FLAG-topo II{alpha} plasmid, lane B is FLAG-topo II{alpha} plasmid 1054-1066, lane C transfected with FLAG-topo II{alpha} plasmid 1017-1028, and lane D is a non-transfected control.