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Fig. 4. FLAG-topo II{alpha} immunofluorescence. Human multiple myeloma H929 cells were transfected by electroporation with full-length wild-type and mutated topo II{alpha} and plated for 20 hours at log and plateau cell densities. Cytospins containing fixed cells were stained with FITC-labeled anti-FLAG M2 antibody (green), counterstained with DAPI for nuclear staining, and assayed by immunofluorescent microscopy.