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Fig. 5. Nuclear export of wild-type and mutant FLAG-topo II{alpha} plasmids. Human myeloma H929 transfected cells (n=20) stained with anti-FLAG M2 monoclonal antibody-FITC conjugate were assayed for nuclear and cytoplasmic immunofluorescence. Quantitation of FITC fluorescence was performed using Adobe Photoshop 7.0 program. Wild-type FLAG-topo II{alpha} was exported to the cytoplasm in cells at plateau density (P=0.00001), whereas topo II{alpha} mutated at the putative export sites, 1016-1027 and 1053-1066, did not demonstrate statistically significant levels of export to the cytoplasm.