Fig. 4. Immunofluorescence microscopy images of syndapin isoforms I, II and III in different cell types. (A) Rat hippocampal neurons in culture immunostained for syndapin I (green) and for the synaptic vesicle marker synaptophysin (red); merged confocal image, colocalization appears yellow. Reproduced with permission from The American Society for Cell Biology (Qualmann et al., 1999). (B,C) Isolated rabbit lacrimal acini (lumen marked by asterisks) treated with the cytoskeletal toxin cytochalasin D display an accumulation of syndapin II (B) close to the actin-rich (C) apical membrane; confocal images. Reproduced with permission from The American Society for Cell Biology (da Costa et al., 2003). (D) Differentiated C2F3 myotubes immunostained for the syndapin III isoform (image kindly provided by M. Plomann).