Fig. 1. Overexpression of EGFP-rab11S25N inhibits ß₂AR recycling from vesicles devoid of EEA1. 12ß6 cells transiently expressing empty EGFP vector (a,a'), EGFP-rab11S25N (b,b'), or EGFP-rab11WT (c,c') were treated with ISO for 20 minutes to induce ß₂AR internalization. They were then washed and placed in media containing propranolol (5 µM) for 15 minutes (A) or 30 minutes (B) at 37°C to allow receptor recycling. ß₂ARs were identified using an anti-C-terminal antibody and EEA1 was identified using a mouse monoclonal antibody. Panels a-c show the localization of ß₂ARs (red) and EEA1 (green), with areas of colocalization appearing yellow, whereas a'-c' show the distribution of EGFP. Scale bar, 10 µm.