Fig. 6. Overexpression of EGFP-rab11WT inhibits ß₂AR recycling from the RE. (A,B) 12ß6 cells transiently overexpressing empty vector (a,a') or EGFP-rab11WT (b,b') were treated with ISO (5 µM) for 6 hours, washed, and receptors were allowed to recycle in the presence of propranolol (5 µM) for 15 minutes (A) or 30 minutes (B) at 37°C. ß₂ARs and EEA1 were labeled as described above and appear red and green, respectively. Arrows in panels b indicate areas of ß₂AR and EGFP-rab11WT colocalization. Scale bars, 10 µm. (C) EcR293: ß₂AR cells transfected with EGFP-rab11WT were treated with either ponasterone () or vehicle () for 72 hours to induce rab11 overexpression, then exposed to ISO for 6 hours. Cells were washed and incubated in DME-H at 37°C for varying times up to 90 minutes to allow receptor recycling and surface receptors were determined as described above. Each point represents the mean±s.e.m. of three independent experiments.