Fig. 1. Expression of Syt V and Syt IX in insulin-secreting cells. (A) PCR amplification of Syt IX cDNA from rat brain, INS-1E cells and rat islets. PCR reactions in which reverse transcriptase was omitted are shown by (–). The DNA size markers (bp) are also indicated. (B) Homogenates (100 µg) of rat brain, INS-1E cells and rat islets were separated by SDS-PAGE and subjected to immunoblotting with anti-Syt V or anti-Syt IX antibodies.