Fig. 8. Immunolabelling of Syt V and Syt IX suppression by RNAi in INS-1E cells. INS-1E cells were transiently transfected with GFP together with the Syt V silencer (A) or with the Syt IX silencer (B). After three days the cells were fixed and analysed by immunofluorescence with the antibodies against Syt V or Syt IX. Transfected cells were identified using GFP fluorescence. The arrows indicate the position of transiently transfected cells expressing the silencer.