Fig. 3. RT-PCR of C166 and HMEC-1 cells grown on matrigel in growth factor containing EGM-2 medium shows DP gene expression at 12 and 24 hours in C166 cells. No DP was detected in C166 cells grown in monolayers in DMEM medium (without growth factors). Tubulin primers were used on the same time 0, C166 mRNA used in lane 1 to show presence of mRNA. +, with reverse transcriptase; –, no reverse transcriptase.